Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn’s Disease and Ulcerative Colitis
Genetic research has shaped the inflammatory bowel disease (IBD) landscape identifying nearly two hundred risk loci. Nonetheless, the identified variants rendered only a partial success in providing criteria for the differential diagnosis between ulcerative colitis (UC) and Crohn’s disease (CD). Tra...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/9208274 |
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| _version_ | 1832552821018329088 |
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| author | Maria Dobre Elena Milanesi Teodora Ecaterina Mănuc Dorel Eugen Arsene Cristian George Ţieranu Carlo Maj Gabriel Becheanu Mircea Mănuc |
| author_facet | Maria Dobre Elena Milanesi Teodora Ecaterina Mănuc Dorel Eugen Arsene Cristian George Ţieranu Carlo Maj Gabriel Becheanu Mircea Mănuc |
| author_sort | Maria Dobre |
| collection | DOAJ |
| description | Genetic research has shaped the inflammatory bowel disease (IBD) landscape identifying nearly two hundred risk loci. Nonetheless, the identified variants rendered only a partial success in providing criteria for the differential diagnosis between ulcerative colitis (UC) and Crohn’s disease (CD). Transcript levels from affected intestinal mucosa may serve as tentative biomarkers for improving classification and diagnosis of IBD. The aim of our study was to identify gene expression profiles specific for UC and CD, in endoscopically affected and normal intestinal colonic mucosa from IBD patients. We evaluated a panel of 84 genes related to the IBD-inflammatory pathway on 21 UC and 22 CD paired inflamed and not inflamed mucosa and on age-matched normal mucosa from 21 non-IBD controls. Two genes in UC (CCL11 and MMP10) and two in CD (C4BPB and IL1RN) showed an upregulation trend in both noninflamed and inflamed mucosa compared to controls. Our results suggest that the transcript levels of CCL11, MMP10, C4BPB, and IL1RN are candidate biomarkers that could help in clinical practice for the differential diagnosis between UC and CD and could guide new research on future therapeutic targets. |
| format | Article |
| id | doaj-art-00d88bace84246aeaf4f4dacd43486e1 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-00d88bace84246aeaf4f4dacd43486e12025-02-03T05:57:36ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/92082749208274Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn’s Disease and Ulcerative ColitisMaria Dobre0Elena Milanesi1Teodora Ecaterina Mănuc2Dorel Eugen Arsene3Cristian George Ţieranu4Carlo Maj5Gabriel Becheanu6Mircea Mănuc7Victor Babes National Institute of Pathology, 050096 Bucharest, RomaniaVictor Babes National Institute of Pathology, 050096 Bucharest, RomaniaFundeni Clinical Institute, 022328 Bucharest, RomaniaVictor Babes National Institute of Pathology, 050096 Bucharest, RomaniaCarol Davila University of Medicine and Pharmacy, 050474 Bucharest, RomaniaInstitute for Genomic Statistics and Bioinformatics, University Hospital Bonn, GermanyFundeni Clinical Institute, 022328 Bucharest, RomaniaFundeni Clinical Institute, 022328 Bucharest, RomaniaGenetic research has shaped the inflammatory bowel disease (IBD) landscape identifying nearly two hundred risk loci. Nonetheless, the identified variants rendered only a partial success in providing criteria for the differential diagnosis between ulcerative colitis (UC) and Crohn’s disease (CD). Transcript levels from affected intestinal mucosa may serve as tentative biomarkers for improving classification and diagnosis of IBD. The aim of our study was to identify gene expression profiles specific for UC and CD, in endoscopically affected and normal intestinal colonic mucosa from IBD patients. We evaluated a panel of 84 genes related to the IBD-inflammatory pathway on 21 UC and 22 CD paired inflamed and not inflamed mucosa and on age-matched normal mucosa from 21 non-IBD controls. Two genes in UC (CCL11 and MMP10) and two in CD (C4BPB and IL1RN) showed an upregulation trend in both noninflamed and inflamed mucosa compared to controls. Our results suggest that the transcript levels of CCL11, MMP10, C4BPB, and IL1RN are candidate biomarkers that could help in clinical practice for the differential diagnosis between UC and CD and could guide new research on future therapeutic targets.http://dx.doi.org/10.1155/2018/9208274 |
| spellingShingle | Maria Dobre Elena Milanesi Teodora Ecaterina Mănuc Dorel Eugen Arsene Cristian George Ţieranu Carlo Maj Gabriel Becheanu Mircea Mănuc Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn’s Disease and Ulcerative Colitis Journal of Immunology Research |
| title | Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn’s Disease and Ulcerative Colitis |
| title_full | Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn’s Disease and Ulcerative Colitis |
| title_fullStr | Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn’s Disease and Ulcerative Colitis |
| title_full_unstemmed | Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn’s Disease and Ulcerative Colitis |
| title_short | Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn’s Disease and Ulcerative Colitis |
| title_sort | differential intestinal mucosa transcriptomic biomarkers for crohn s disease and ulcerative colitis |
| url | http://dx.doi.org/10.1155/2018/9208274 |
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