Histological Characterization of the Dicer1 Mutant Zebrafish Retina
DICER1, a multidomain RNase III endoribonuclease, plays a critical role in microRNA (miRNA) and RNA-interference (RNAi) functional pathways. Loss of Dicer1 affects different developmental processes. Dicer1 is essential for retinal development and maintenance. DICER1 was recently shown to have anothe...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/309510 |
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| author | Saeed Akhtar Sarita Rani Patnaik Rakesh Kotapati Raghupathy Turki M. Al-Mubrad John A. Craft Xinhua Shu |
| author_facet | Saeed Akhtar Sarita Rani Patnaik Rakesh Kotapati Raghupathy Turki M. Al-Mubrad John A. Craft Xinhua Shu |
| author_sort | Saeed Akhtar |
| collection | DOAJ |
| description | DICER1, a multidomain RNase III endoribonuclease, plays a critical role in microRNA (miRNA) and RNA-interference (RNAi) functional pathways. Loss of Dicer1 affects different developmental processes. Dicer1 is essential for retinal development and maintenance. DICER1 was recently shown to have another function of silencing the toxicity of Alu RNAs in retinal pigment epithelium (RPE) cells, which are involved in the pathogenesis of age related macular degeneration. In this study, we characterized a Dicer1 mutant fish line, which carries a nonsense mutation (W1457Ter) induced by N-ethyl-N-nitrosourea mutagenesis. Zebrafish DICER1 protein is highly conserved in the evolution. Zebrafish Dicer1 is expressed at the earliest stages of zebrafish development and persists into late developmental stages; it is widely expressed in adult tissues. Homozygous Dicer1 mutant fish (DICER1W1457Ter/W1457Ter) have an arrest in early growth with significantly smaller eyes and are dead at 14–18 dpf. Heterozygous Dicer1 mutant fish have similar retinal structure to that of control fish; the retinal pigment epithelium (RPE) cells are normal with no sign of degeneration at the age of 20 months. |
| format | Article |
| id | doaj-art-00d63ed3b5664768b69e07820ba92ea7 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-00d63ed3b5664768b69e07820ba92ea72025-02-03T06:13:43ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/309510309510Histological Characterization of the Dicer1 Mutant Zebrafish RetinaSaeed Akhtar0Sarita Rani Patnaik1Rakesh Kotapati Raghupathy2Turki M. Al-Mubrad3John A. Craft4Xinhua Shu5Cornea Research Chair, Department of Optometry, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi ArabiaDepartment of Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKDepartment of Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKCornea Research Chair, Department of Optometry, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi ArabiaDepartment of Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKDepartment of Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKDICER1, a multidomain RNase III endoribonuclease, plays a critical role in microRNA (miRNA) and RNA-interference (RNAi) functional pathways. Loss of Dicer1 affects different developmental processes. Dicer1 is essential for retinal development and maintenance. DICER1 was recently shown to have another function of silencing the toxicity of Alu RNAs in retinal pigment epithelium (RPE) cells, which are involved in the pathogenesis of age related macular degeneration. In this study, we characterized a Dicer1 mutant fish line, which carries a nonsense mutation (W1457Ter) induced by N-ethyl-N-nitrosourea mutagenesis. Zebrafish DICER1 protein is highly conserved in the evolution. Zebrafish Dicer1 is expressed at the earliest stages of zebrafish development and persists into late developmental stages; it is widely expressed in adult tissues. Homozygous Dicer1 mutant fish (DICER1W1457Ter/W1457Ter) have an arrest in early growth with significantly smaller eyes and are dead at 14–18 dpf. Heterozygous Dicer1 mutant fish have similar retinal structure to that of control fish; the retinal pigment epithelium (RPE) cells are normal with no sign of degeneration at the age of 20 months.http://dx.doi.org/10.1155/2015/309510 |
| spellingShingle | Saeed Akhtar Sarita Rani Patnaik Rakesh Kotapati Raghupathy Turki M. Al-Mubrad John A. Craft Xinhua Shu Histological Characterization of the Dicer1 Mutant Zebrafish Retina Journal of Ophthalmology |
| title | Histological Characterization of the Dicer1 Mutant Zebrafish Retina |
| title_full | Histological Characterization of the Dicer1 Mutant Zebrafish Retina |
| title_fullStr | Histological Characterization of the Dicer1 Mutant Zebrafish Retina |
| title_full_unstemmed | Histological Characterization of the Dicer1 Mutant Zebrafish Retina |
| title_short | Histological Characterization of the Dicer1 Mutant Zebrafish Retina |
| title_sort | histological characterization of the dicer1 mutant zebrafish retina |
| url | http://dx.doi.org/10.1155/2015/309510 |
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