Investigating the Effect of Enzymatically-Derived Blackcurrant Extract on Skin Staphylococci Using an In Vitro Human <i>Stratum Corneum</i> Model
<b>Background/Objectives</b>: Numerous intrinsic and extrinsic stressors can disrupt the balance of the skin microbiome, leading to the development of various skin diseases. It has been proven that coagulase-negative staphylococci (CoNS) are important commensals for maintaining skin micr...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/17/4/487 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849714273139294208 |
|---|---|
| author | Marija Ćorović Anja Petrov Ivanković Ana Milivojević Klaus Pfeffer Bernhard Homey Patrick A. M. Jansen Patrick L. J. M. Zeeuwen Ellen H. van den Bogaard Dejan Bezbradica |
| author_facet | Marija Ćorović Anja Petrov Ivanković Ana Milivojević Klaus Pfeffer Bernhard Homey Patrick A. M. Jansen Patrick L. J. M. Zeeuwen Ellen H. van den Bogaard Dejan Bezbradica |
| author_sort | Marija Ćorović |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Numerous intrinsic and extrinsic stressors can disrupt the balance of the skin microbiome, leading to the development of various skin diseases. It has been proven that coagulase-negative staphylococci (CoNS) are important commensals for maintaining skin microbiome homeostasis and fighting cutaneous pathogens such as <i>Staphylococcus aureus</i> (<i>S. aureus</i>). Here, we examined the influence of polyphenol-rich enzymatic blackcurrant extract (EBCE) on pathogenic coagulase-positive <i>S. aureus</i> strains and beneficial CoNS, like <i>Staphylococcus epidermidis</i> (<i>S. epidermidis</i>), to explore its potential for rebalancing the skin microbiota. <b>Methods</b>: The polyphenol profile of EBCE was determined by ultra-high-pressure liquid chromatography–tandem mass spectrometry. Microwell plate assays were employed to study the effect of EBCE on five <i>S. aureus</i> strains isolated from the skin of atopic dermatitis patients. An in vitro human <i>stratum corneum</i> model was used to test its effect on mixed bacterial cultures. <b>Results</b>: EBCE inhibited the growth of all tested <i>S. aureus</i> strains by 80–100% at the highest tested concentration after 7 h. No microbial growth was observed at the highest tested EBCE concentration using the <i>stratum corneum</i> model inoculated with one selected pathogen (<i>S. aureus</i> SA-DUS-017) and one commensal laboratory strain (<i>S. epidermidis</i> DSM 20044). The lowest tested concentration did not interfere with <i>S. aureus</i> growth but strongly stimulated the growth of <i>S. epidermidis</i> (~300-fold colony forming unit increase). In addition, low EBCE concentrations strongly stimulated CoNS growth in microbiome samples taken from the armpits of healthy volunteers that were spiked with <i>S. aureus</i> SA-DUS-017. <b>Conclusions</b>: These preclinical data support further testing of EBCE-enriched topical preparations as potential cutaneous prebiotics in human studies. |
| format | Article |
| id | doaj-art-00a4dcb819a84f65bb92e15cf83421c9 |
| institution | DOAJ |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-00a4dcb819a84f65bb92e15cf83421c92025-08-20T03:13:45ZengMDPI AGPharmaceutics1999-49232025-04-0117448710.3390/pharmaceutics17040487Investigating the Effect of Enzymatically-Derived Blackcurrant Extract on Skin Staphylococci Using an In Vitro Human <i>Stratum Corneum</i> ModelMarija Ćorović0Anja Petrov Ivanković1Ana Milivojević2Klaus Pfeffer3Bernhard Homey4Patrick A. M. Jansen5Patrick L. J. M. Zeeuwen6Ellen H. van den Bogaard7Dejan Bezbradica8Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, SerbiaInnovation Center, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, SerbiaFaculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, SerbiaDepartment of Microbiology, University Hospital Düsseldorf, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, GermanyDepartment of Dermatology, University Hospital Düsseldorf, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, GermanyDepartment of Dermatology, Radboud University Medical Center, 6525 GA Nijmegen, The NetherlandsDepartment of Dermatology, Radboud University Medical Center, 6525 GA Nijmegen, The NetherlandsDepartment of Dermatology, Radboud University Medical Center, 6525 GA Nijmegen, The NetherlandsFaculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia<b>Background/Objectives</b>: Numerous intrinsic and extrinsic stressors can disrupt the balance of the skin microbiome, leading to the development of various skin diseases. It has been proven that coagulase-negative staphylococci (CoNS) are important commensals for maintaining skin microbiome homeostasis and fighting cutaneous pathogens such as <i>Staphylococcus aureus</i> (<i>S. aureus</i>). Here, we examined the influence of polyphenol-rich enzymatic blackcurrant extract (EBCE) on pathogenic coagulase-positive <i>S. aureus</i> strains and beneficial CoNS, like <i>Staphylococcus epidermidis</i> (<i>S. epidermidis</i>), to explore its potential for rebalancing the skin microbiota. <b>Methods</b>: The polyphenol profile of EBCE was determined by ultra-high-pressure liquid chromatography–tandem mass spectrometry. Microwell plate assays were employed to study the effect of EBCE on five <i>S. aureus</i> strains isolated from the skin of atopic dermatitis patients. An in vitro human <i>stratum corneum</i> model was used to test its effect on mixed bacterial cultures. <b>Results</b>: EBCE inhibited the growth of all tested <i>S. aureus</i> strains by 80–100% at the highest tested concentration after 7 h. No microbial growth was observed at the highest tested EBCE concentration using the <i>stratum corneum</i> model inoculated with one selected pathogen (<i>S. aureus</i> SA-DUS-017) and one commensal laboratory strain (<i>S. epidermidis</i> DSM 20044). The lowest tested concentration did not interfere with <i>S. aureus</i> growth but strongly stimulated the growth of <i>S. epidermidis</i> (~300-fold colony forming unit increase). In addition, low EBCE concentrations strongly stimulated CoNS growth in microbiome samples taken from the armpits of healthy volunteers that were spiked with <i>S. aureus</i> SA-DUS-017. <b>Conclusions</b>: These preclinical data support further testing of EBCE-enriched topical preparations as potential cutaneous prebiotics in human studies.https://www.mdpi.com/1999-4923/17/4/487skin prebioticblackcurrant extract<i>Staphylococcus aureus</i>coagulase-negative staphylococci<i>stratum corneum</i> model |
| spellingShingle | Marija Ćorović Anja Petrov Ivanković Ana Milivojević Klaus Pfeffer Bernhard Homey Patrick A. M. Jansen Patrick L. J. M. Zeeuwen Ellen H. van den Bogaard Dejan Bezbradica Investigating the Effect of Enzymatically-Derived Blackcurrant Extract on Skin Staphylococci Using an In Vitro Human <i>Stratum Corneum</i> Model Pharmaceutics skin prebiotic blackcurrant extract <i>Staphylococcus aureus</i> coagulase-negative staphylococci <i>stratum corneum</i> model |
| title | Investigating the Effect of Enzymatically-Derived Blackcurrant Extract on Skin Staphylococci Using an In Vitro Human <i>Stratum Corneum</i> Model |
| title_full | Investigating the Effect of Enzymatically-Derived Blackcurrant Extract on Skin Staphylococci Using an In Vitro Human <i>Stratum Corneum</i> Model |
| title_fullStr | Investigating the Effect of Enzymatically-Derived Blackcurrant Extract on Skin Staphylococci Using an In Vitro Human <i>Stratum Corneum</i> Model |
| title_full_unstemmed | Investigating the Effect of Enzymatically-Derived Blackcurrant Extract on Skin Staphylococci Using an In Vitro Human <i>Stratum Corneum</i> Model |
| title_short | Investigating the Effect of Enzymatically-Derived Blackcurrant Extract on Skin Staphylococci Using an In Vitro Human <i>Stratum Corneum</i> Model |
| title_sort | investigating the effect of enzymatically derived blackcurrant extract on skin staphylococci using an in vitro human i stratum corneum i model |
| topic | skin prebiotic blackcurrant extract <i>Staphylococcus aureus</i> coagulase-negative staphylococci <i>stratum corneum</i> model |
| url | https://www.mdpi.com/1999-4923/17/4/487 |
| work_keys_str_mv | AT marijacorovic investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel AT anjapetrovivankovic investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel AT anamilivojevic investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel AT klauspfeffer investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel AT bernhardhomey investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel AT patrickamjansen investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel AT patrickljmzeeuwen investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel AT ellenhvandenbogaard investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel AT dejanbezbradica investigatingtheeffectofenzymaticallyderivedblackcurrantextractonskinstaphylococciusinganinvitrohumanistratumcorneumimodel |