In vivo versus in vitro sperm selection
The separation of human spermatozoa is an important step in therapy of human infertility. Given that male fertility is decreasing and, as a result, fertilization techniques based on microinjection of sperm into the cytoplasm are being used more intensively, this question is becoming increasingly...
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| Format: | Article |
| Language: | English |
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MRE Press
2025-02-01
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| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/files/article/20250228-502/pdf/JOMH2024101802.pdf |
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| author | Michal Ješeta Jana Antalíková Adéla Doubravská Lenka Mekiňová Bartosz Kempisty Pavel Ventruba Igor Crha |
| author_facet | Michal Ješeta Jana Antalíková Adéla Doubravská Lenka Mekiňová Bartosz Kempisty Pavel Ventruba Igor Crha |
| author_sort | Michal Ješeta |
| collection | DOAJ |
| description | The separation of human spermatozoa is an important step in therapy of human
infertility. Given that male fertility is decreasing and, as a result,
fertilization techniques based on microinjection of sperm into the cytoplasm are
being used more intensively, this question is becoming increasingly relevant. In
recent years, microfluidic sperm processing techniques have been increasingly
used. These methods are simple and easy to use, however, the question is to what
extent they select the correct sperm. They are essentially based mainly on
motility and do not reflect other navigational approaches such as chemotaxis,
thermotaxis or rheotaxis. This review compares traditional, advances and
novel in vitro methods of sperm separation which are commonly used
during human infertility therapy in context of in vivo sperm separation
in female reproductive system. |
| format | Article |
| id | doaj-art-00a3e47258b84a45a464b1b024c8d795 |
| institution | DOAJ |
| issn | 1875-6867 1875-6859 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MRE Press |
| record_format | Article |
| series | Journal of Men's Health |
| spelling | doaj-art-00a3e47258b84a45a464b1b024c8d7952025-08-20T03:16:35ZengMRE PressJournal of Men's Health1875-68671875-68592025-02-0121211010.22514/jomh.2025.016S1875-6867(25)00333-1In vivo versus in vitro sperm selectionMichal Ješeta0Jana Antalíková1Adéla Doubravská2Lenka Mekiňová3Bartosz Kempisty4Pavel Ventruba5Igor Crha6Department of Gynecology and Obstetrics, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, 62500 Brno, Czech Republic Laboratory of Reproductive Physiology, Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, 84005 Bratislava, Slovak Republic Department of Gynecology and Obstetrics, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, 62500 Brno, Czech Republic Department of Gynecology and Obstetrics, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, 62500 Brno, Czech Republic Department of Veterinary Medicine, Nicolaus Copernicus University, 87-100 Torun, Poland Department of Gynecology and Obstetrics, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, 62500 Brno, Czech Republic Department of Gynecology and Obstetrics, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, 62500 Brno, Czech Republic The separation of human spermatozoa is an important step in therapy of human infertility. Given that male fertility is decreasing and, as a result, fertilization techniques based on microinjection of sperm into the cytoplasm are being used more intensively, this question is becoming increasingly relevant. In recent years, microfluidic sperm processing techniques have been increasingly used. These methods are simple and easy to use, however, the question is to what extent they select the correct sperm. They are essentially based mainly on motility and do not reflect other navigational approaches such as chemotaxis, thermotaxis or rheotaxis. This review compares traditional, advances and novel in vitro methods of sperm separation which are commonly used during human infertility therapy in context of in vivo sperm separation in female reproductive system.https://oss.jomh.org/files/article/20250228-502/pdf/JOMH2024101802.pdfspermatozoaivfsperm separationicsimicrofluid separationthermotaxischemotaxis |
| spellingShingle | Michal Ješeta Jana Antalíková Adéla Doubravská Lenka Mekiňová Bartosz Kempisty Pavel Ventruba Igor Crha In vivo versus in vitro sperm selection Journal of Men's Health spermatozoa ivf sperm separation icsi microfluid separation thermotaxis chemotaxis |
| title | In vivo versus in vitro sperm selection |
| title_full | In vivo versus in vitro sperm selection |
| title_fullStr | In vivo versus in vitro sperm selection |
| title_full_unstemmed | In vivo versus in vitro sperm selection |
| title_short | In vivo versus in vitro sperm selection |
| title_sort | in vivo versus in vitro sperm selection |
| topic | spermatozoa ivf sperm separation icsi microfluid separation thermotaxis chemotaxis |
| url | https://oss.jomh.org/files/article/20250228-502/pdf/JOMH2024101802.pdf |
| work_keys_str_mv | AT michaljeseta invivoversusinvitrospermselection AT janaantalikova invivoversusinvitrospermselection AT adeladoubravska invivoversusinvitrospermselection AT lenkamekinova invivoversusinvitrospermselection AT bartoszkempisty invivoversusinvitrospermselection AT pavelventruba invivoversusinvitrospermselection AT igorcrha invivoversusinvitrospermselection |