Assessment of Direct RT-qPCR for SARS-CoV-2 Detection by Multiplexed Assay

Assessment of Direct RT-qPCR for SARS-CoV-2 Detection by Multiplexed Assay

Abstract RNA extraction is usually required for molecular detection methods. On the other hand, this step implies time consumption and additional cost to laboratorial routine. This study evaluated the use of direct-RT-qPCR for SARS-CoV-2 detection compared to the gold standard extraction method. An...

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Bibliographic Details
Main Authors: Rachel Cruz Alves, José Henrique Franscisco Roma, Bruno Moreira Carneiro, Renata Dezengrini Slhessarenko, Juliana Helena Chavez Pavoni
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2025-05-01
Series:Brazilian Archives of Biology and Technology
Subjects:
COVID-19 Nucleic Acid Testing
SARS-CoV-2
Molecular Diagnosis
Sensitivity and Specificity.
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132025000100306&lng=en&tlng=en
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