Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucose
Objective To investigate the effect and possible mechanisms of angiotensin Ⅱ type1 receptor(AT1 R) on injury of renal tubular epithelial cells induced by high glucose. Methods The expression of AT1 R,AdipoR1,NF-κB,MCP-1,MIP-1 α,α-SMA,FN were detected by using western blot. The colocalization of AT1...
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Editorial Department of Journal of Clinical Nephrology
2018-01-01
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| Series: | Linchuang shenzangbing zazhi |
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| author | ZHA Dong-qing YAO Tao GAO Ping WU Xiao-yan |
| author_facet | ZHA Dong-qing YAO Tao GAO Ping WU Xiao-yan |
| author_sort | ZHA Dong-qing |
| collection | DOAJ |
| description | Objective To investigate the effect and possible mechanisms of angiotensin Ⅱ type1 receptor(AT1 R) on injury of renal tubular epithelial cells induced by high glucose. Methods The expression of AT1 R,AdipoR1,NF-κB,MCP-1,MIP-1 α,α-SMA,FN were detected by using western blot. The colocalization of AT1 R and AdipoR1 was detected by confocal microscopy. The dimerization of AT1 R and AdipoR1 was detected by immunoprecipitation. Results Compared with control group,the expression of AdipoR1 had no significant change and the expression of AT1 R was increased in rat renal tubular epithelial cells induced by high glucose. The expression of inflammatory factors as NF-κB,MCP-1 and MIP-1 α was increased in rat renal tubular epithelial cells stimulated with high glucose.Compared with control group,the expression of α-SMA and FN was increased in rat renal tubular epithelial cells stimulated with high glucose. The dimerization of AT1 R and AdipoR1 was formed in the rat renal tubular epithelial cells. And the AT1 R-adipoR1 dimerization was increased in the rat renal tubular epithelial cells induced by high glucose. Knockdown of AT1 R by siRNA inhibited the demerization of AT1 R-adipoR1,reduced the expression of inflammatory factors as NF-κB,MCP-1 and MIP-1 α,decreased the expression of α-SMA and FN in rat renal tubular epithelial cells induced by high glucose.Conclusions AT1 R promotes the inflammation and fibrosis of renal tubular epithelial cells induced by high glucose through dimerization of AT1 R-adipoR1. |
| format | Article |
| id | doaj-art-0085450c80244b7cac0c7cf4b0d3eca4 |
| institution | OA Journals |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2018-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-0085450c80244b7cac0c7cf4b0d3eca42025-08-20T01:51:07ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902018-01-0142943357914718Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucoseZHA Dong-qingYAO TaoGAO PingWU Xiao-yanObjective To investigate the effect and possible mechanisms of angiotensin Ⅱ type1 receptor(AT1 R) on injury of renal tubular epithelial cells induced by high glucose. Methods The expression of AT1 R,AdipoR1,NF-κB,MCP-1,MIP-1 α,α-SMA,FN were detected by using western blot. The colocalization of AT1 R and AdipoR1 was detected by confocal microscopy. The dimerization of AT1 R and AdipoR1 was detected by immunoprecipitation. Results Compared with control group,the expression of AdipoR1 had no significant change and the expression of AT1 R was increased in rat renal tubular epithelial cells induced by high glucose. The expression of inflammatory factors as NF-κB,MCP-1 and MIP-1 α was increased in rat renal tubular epithelial cells stimulated with high glucose.Compared with control group,the expression of α-SMA and FN was increased in rat renal tubular epithelial cells stimulated with high glucose. The dimerization of AT1 R and AdipoR1 was formed in the rat renal tubular epithelial cells. And the AT1 R-adipoR1 dimerization was increased in the rat renal tubular epithelial cells induced by high glucose. Knockdown of AT1 R by siRNA inhibited the demerization of AT1 R-adipoR1,reduced the expression of inflammatory factors as NF-κB,MCP-1 and MIP-1 α,decreased the expression of α-SMA and FN in rat renal tubular epithelial cells induced by high glucose.Conclusions AT1 R promotes the inflammation and fibrosis of renal tubular epithelial cells induced by high glucose through dimerization of AT1 R-adipoR1.http://www.lcszb.com/thesisDetails?columnId=57914718&Fpath=home&index=0Angiotensin Ⅱ type 1 receptorAdiponectin receptor 1DimerizationRenal tubular epithelial cellsInflammation |
| spellingShingle | ZHA Dong-qing YAO Tao GAO Ping WU Xiao-yan Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucose Linchuang shenzangbing zazhi Angiotensin Ⅱ type 1 receptor Adiponectin receptor 1 Dimerization Renal tubular epithelial cells Inflammation |
| title | Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucose |
| title_full | Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucose |
| title_fullStr | Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucose |
| title_full_unstemmed | Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucose |
| title_short | Effect of angiotensin Ⅱ type 1 receptor on injury of renal tubular epithelial cells induced by high glucose |
| title_sort | effect of angiotensin ii type 1 receptor on injury of renal tubular epithelial cells induced by high glucose |
| topic | Angiotensin Ⅱ type 1 receptor Adiponectin receptor 1 Dimerization Renal tubular epithelial cells Inflammation |
| url | http://www.lcszb.com/thesisDetails?columnId=57914718&Fpath=home&index=0 |
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