Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis
This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gum–alginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption inc...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2016-07-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1177/0263617416659287 |
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| _version_ | 1832544659664011264 |
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| author | Ana C Santos Leite da Silva Nadia Woitovich Valetti María E Brassesco José A Teixeira Guillermo Picó |
| author_facet | Ana C Santos Leite da Silva Nadia Woitovich Valetti María E Brassesco José A Teixeira Guillermo Picó |
| author_sort | Ana C Santos Leite da Silva |
| collection | DOAJ |
| description | This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gum–alginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order rate kinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix. |
| format | Article |
| id | doaj-art-0064a80cd6ea4a7c8157b28fe97ac925 |
| institution | Kabale University |
| issn | 0263-6174 2048-4038 |
| language | English |
| publishDate | 2016-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj-art-0064a80cd6ea4a7c8157b28fe97ac9252025-02-03T10:07:35ZengSAGE PublishingAdsorption Science & Technology0263-61742048-40382016-07-013410.1177/0263617416659287Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysisAna C Santos Leite da SilvaNadia Woitovich ValettiMaría E BrassescoJosé A TeixeiraGuillermo PicóThis work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gum–alginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order rate kinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix.https://doi.org/10.1177/0263617416659287 |
| spellingShingle | Ana C Santos Leite da Silva Nadia Woitovich Valetti María E Brassesco José A Teixeira Guillermo Picó Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis Adsorption Science & Technology |
| title | Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis |
| title_full | Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis |
| title_fullStr | Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis |
| title_full_unstemmed | Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis |
| title_short | Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis |
| title_sort | adsorption of peroxidase from l onto alginate guar gum matrix kinetic equilibrium and thermodynamic analysis |
| url | https://doi.org/10.1177/0263617416659287 |
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