Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2
The development of accurate and high-throughput tools for cancer biomarker detection is crucial for the diagnosis, monitoring, and treatment of diseases. In this study, we developed a simple and rapid fluorescence-linked immunosorbent assay (FLISA) using fluorescent dye-conjugated antibody fragments...
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MDPI AG
2025-01-01
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| author | Ji Eun Choi Hanool Yun Hee-Jin Jeong |
| author_facet | Ji Eun Choi Hanool Yun Hee-Jin Jeong |
| author_sort | Ji Eun Choi |
| collection | DOAJ |
| description | The development of accurate and high-throughput tools for cancer biomarker detection is crucial for the diagnosis, monitoring, and treatment of diseases. In this study, we developed a simple and rapid fluorescence-linked immunosorbent assay (FLISA) using fluorescent dye-conjugated antibody fragments against programmed cell death ligand 1 (PDL1) and human epithelial growth factor receptor 2 (HER2). We optimized key steps in the FLISA process, including antigen immobilization, blocking, and antibody reaction, reading the assay time to 3 h—significantly faster compared to the 23 h duration of usual FLISA. The limit of detection for the rapid FLISA in detecting PDL1 was lower than that of FLISA, and the detection of HER2 was similar between the two methods, indicating that the rapid FLISA provides a fast and accurate approach for detecting PDL1 and HER2. This robust platform can be readily adapted for various fluoroimmunoassays targeting other antigens of interest. |
| format | Article |
| id | doaj-art-002660a2a75445adb54e9c7b0301a59a |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-002660a2a75445adb54e9c7b0301a59a2025-01-24T13:27:36ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-01-014716210.3390/cimb47010062Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2Ji Eun Choi0Hanool Yun1Hee-Jin Jeong2Department of Pathology, Chungnam National University Sejong Hospital, Sejong 30099, Republic of KoreaDepartment of Biological and Chemical Engineering, Hongik University, Sejong 30016, Republic of KoreaDepartment of Biological and Chemical Engineering, Hongik University, Sejong 30016, Republic of KoreaThe development of accurate and high-throughput tools for cancer biomarker detection is crucial for the diagnosis, monitoring, and treatment of diseases. In this study, we developed a simple and rapid fluorescence-linked immunosorbent assay (FLISA) using fluorescent dye-conjugated antibody fragments against programmed cell death ligand 1 (PDL1) and human epithelial growth factor receptor 2 (HER2). We optimized key steps in the FLISA process, including antigen immobilization, blocking, and antibody reaction, reading the assay time to 3 h—significantly faster compared to the 23 h duration of usual FLISA. The limit of detection for the rapid FLISA in detecting PDL1 was lower than that of FLISA, and the detection of HER2 was similar between the two methods, indicating that the rapid FLISA provides a fast and accurate approach for detecting PDL1 and HER2. This robust platform can be readily adapted for various fluoroimmunoassays targeting other antigens of interest.https://www.mdpi.com/1467-3045/47/1/62fluorescence-linked immunosorbent assayimmunoassayfluorescent biosensorPDL1HER2 |
| spellingShingle | Ji Eun Choi Hanool Yun Hee-Jin Jeong Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2 Current Issues in Molecular Biology fluorescence-linked immunosorbent assay immunoassay fluorescent biosensor PDL1 HER2 |
| title | Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2 |
| title_full | Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2 |
| title_fullStr | Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2 |
| title_full_unstemmed | Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2 |
| title_short | Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2 |
| title_sort | establishment of a rapid and convenient fluoroimmunoassay platform using antibodies against pdl1 and her2 |
| topic | fluorescence-linked immunosorbent assay immunoassay fluorescent biosensor PDL1 HER2 |
| url | https://www.mdpi.com/1467-3045/47/1/62 |
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