DNA methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativa

BackgroundHidradenitis suppurativa (HS) is a chronic inflammatory skin condition that affects hair follicles in areas with apocrine sweat glands, such as the underarms, groin, and buttocks. The pathogenesis of HS is not fully understood, but considering the key role played by the biological clock in...

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Main Authors: Uppala Radhakrishna, Uppala Ratnamala, Devendrasinh D. Jhala, Lavanya V. Uppala, Aaren Vedangi, Nazia Saiyed, Sushma R. Shah, Maulikkumar Patel, Rakesh M. Rawal, Tommaso Mazza, Gregor B. E. Jemec, Gianluigi Mazzoccoli, Giovanni Damiani
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Immunology
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Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2024.1475424/full
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author Uppala Radhakrishna
Uppala Ratnamala
Devendrasinh D. Jhala
Lavanya V. Uppala
Aaren Vedangi
Nazia Saiyed
Sushma R. Shah
Maulikkumar Patel
Rakesh M. Rawal
Tommaso Mazza
Gregor B. E. Jemec
Gianluigi Mazzoccoli
Giovanni Damiani
Giovanni Damiani
author_facet Uppala Radhakrishna
Uppala Ratnamala
Devendrasinh D. Jhala
Lavanya V. Uppala
Aaren Vedangi
Nazia Saiyed
Sushma R. Shah
Maulikkumar Patel
Rakesh M. Rawal
Tommaso Mazza
Gregor B. E. Jemec
Gianluigi Mazzoccoli
Giovanni Damiani
Giovanni Damiani
author_sort Uppala Radhakrishna
collection DOAJ
description BackgroundHidradenitis suppurativa (HS) is a chronic inflammatory skin condition that affects hair follicles in areas with apocrine sweat glands, such as the underarms, groin, and buttocks. The pathogenesis of HS is not fully understood, but considering the key role played by the biological clock in the control of immune/inflammatory processes the derangement of circadian and ultradian pathways could be hypothesized.MethodsWe analyzed genome-wide DNA methylation patterns in peripheral blood from 24 HS cases and 24 controls using the Infinium HumanMethylation450 BeadChip array (Illumina), followed by bioinformatics and statistical analyses.ResultsWe found that several circadian and ultradian genes were differentially methylated in HS patients, predominantly exhibiting hypomethylation. These genes were enriched in pathways such as MAPK and WNT cascades, acute phase response, cytokine release, inflammation, innate immune response, xenobiotic detoxification, and oxidative stress response.ConclusionAltered DNA methylation patterns of genes related to circadian and ultradian pathways could contribute to immune system derangement and inflammatory processes chronicization in addition to other comorbidities hallmarking HS onset and progression, at the same time representing possible druggable targets.
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spelling doaj-art-000e0ca1b9374a09ab6be67c459041c22025-08-20T01:54:15ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-11-011510.3389/fimmu.2024.14754241475424DNA methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativaUppala Radhakrishna0Uppala Ratnamala1Devendrasinh D. Jhala2Lavanya V. Uppala3Aaren Vedangi4Nazia Saiyed5Sushma R. Shah6Maulikkumar Patel7Rakesh M. Rawal8Tommaso Mazza9Gregor B. E. Jemec10Gianluigi Mazzoccoli11Giovanni Damiani12Giovanni Damiani13Department of Anesthesiology and Perioperative Medicine, University of Pittsburgh, Pittsburgh, PA, United StatesDepartment of Life Sciences, School of Sciences, Gujarat University, Ahmedabad, IndiaDepartment of Zoology, School of Sciences, Gujarat University, Ahmedabad, IndiaCollege of Information Science and Technology, Peter Kiewit Institute, The University of Nebraska at Omaha, Omaha, NE, United StatesDepartment of Clinical Research, KIMS ICON Hospital, A Unit of ICON Krishi Institute Medical Sciences, Visakhapatnam, IndiaDepartment of Obstetrics and Gynecology, Beaumont Health, Royal Oak, MI, United StatesDepartment of Obstetrics and Gynecology, BJ Medical College Institute of Medical Post-Graduate Studies and Research, Ahmedabad, IndiaDepartment of Life Sciences, School of Sciences, Gujarat University, Ahmedabad, IndiaDepartment of Life Sciences, School of Sciences, Gujarat University, Ahmedabad, IndiaBioinformatics Unit, IRCCS “Casa Sollievo della Sofferenza”, Opera di Padre Pio da Pietrelcina, San Giovanni Rotondo, FG, ItalyDepartment of Dermatology, Zealand University Hospital, Roskilde, Denmark0Department of Medical Sciences, Division of Internal Medicine and Chronobiology Laboratory, Fondazione IRCCS “Casa Sollievo della Sofferenza”, San Giovanni Rotondo, FG, Italy1Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy2Italian Center of Precision Medicine and Chronic Inflammation, University of Milan, Milan, ItalyBackgroundHidradenitis suppurativa (HS) is a chronic inflammatory skin condition that affects hair follicles in areas with apocrine sweat glands, such as the underarms, groin, and buttocks. The pathogenesis of HS is not fully understood, but considering the key role played by the biological clock in the control of immune/inflammatory processes the derangement of circadian and ultradian pathways could be hypothesized.MethodsWe analyzed genome-wide DNA methylation patterns in peripheral blood from 24 HS cases and 24 controls using the Infinium HumanMethylation450 BeadChip array (Illumina), followed by bioinformatics and statistical analyses.ResultsWe found that several circadian and ultradian genes were differentially methylated in HS patients, predominantly exhibiting hypomethylation. These genes were enriched in pathways such as MAPK and WNT cascades, acute phase response, cytokine release, inflammation, innate immune response, xenobiotic detoxification, and oxidative stress response.ConclusionAltered DNA methylation patterns of genes related to circadian and ultradian pathways could contribute to immune system derangement and inflammatory processes chronicization in addition to other comorbidities hallmarking HS onset and progression, at the same time representing possible druggable targets.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1475424/fullepigeneticshidradenitis suppurativabiological clockcircadianultradianrhythmicity
spellingShingle Uppala Radhakrishna
Uppala Ratnamala
Devendrasinh D. Jhala
Lavanya V. Uppala
Aaren Vedangi
Nazia Saiyed
Sushma R. Shah
Maulikkumar Patel
Rakesh M. Rawal
Tommaso Mazza
Gregor B. E. Jemec
Gianluigi Mazzoccoli
Giovanni Damiani
Giovanni Damiani
DNA methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativa
Frontiers in Immunology
epigenetics
hidradenitis suppurativa
biological clock
circadian
ultradian
rhythmicity
title DNA methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativa
title_full DNA methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativa
title_fullStr DNA methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativa
title_full_unstemmed DNA methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativa
title_short DNA methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativa
title_sort dna methylation patterns of circadian and ultradian genes are altered in the peripheral blood of patients with hidradenitis suppurativa
topic epigenetics
hidradenitis suppurativa
biological clock
circadian
ultradian
rhythmicity
url https://www.frontiersin.org/articles/10.3389/fimmu.2024.1475424/full
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